› 3cp8

X-ray diffraction
3.2Å resolution

Crystal structure of GidA from Chlorobium tepidum

Released:
DOI: 10.2210/pdb3cp8/pdb
PDB entry 3cp8 coloured by chain, front view.
PDB entry 3cp8 coloured by chain, side view.
PDB entry 3cp8 coloured by chain, top view.
Source organism: Chlorobaculum tepidum TLS
Primary publication:
Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate.
Meyer S, Scrima A, Versées W, Wittinghofer A
J Mol Biol 380 532-47 (2008)
PMID: 18565343

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 641 amino acids
Theoretical weight: 70.36 KDa
Source organism: Chlorobaculum tepidum TLS
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KA85 (Residues: 1-621; Coverage: 100%)
Gene names: CT2283, gidA, mnmG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 120.505Å b: 71.836Å c: 171.934Å
α: 90° β: 91.82° γ: 90°
R-values:
R R work R free
0.256 0.254 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

It takes two to tango: regulation of G proteins by dimerization.
Gasper et al. (2009)
8 more

4 mentions without citation

MTO1 mutations are associated with hypertrophic cardiomyopathy and lactic acidosis and cause respiratory chain deficiency in humans and yeast.
Baruffini et al. (2013)
3 more