› 3ck8

X-ray diffraction
2.1Å resolution

B. thetaiotaomicron SusD with beta-cyclodextrin

Released:
DOI: 10.2210/pdb3ck8/pdb
PDB entry 3ck8 coloured by chain, front view.
PDB entry 3ck8 coloured by chain, side view.
PDB entry 3ck8 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Starch catabolism by a prominent human gut symbiont is directed by the recognition of amylose helices.
Koropatkin NM, Martens EC, Gordon JI, Smith TJ
Structure 16 1105-15 (2008)
PMID: 18611383

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Starch-binding protein SusD Chains: A, B
Molecule details ›
Chains: A, B
Length: 527 amino acids
Theoretical weight: 59.78 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A1G2 (Residues: 26-551; Coverage: 100%)
Gene names: BT_3701, susD
Sequence domains: SusD family
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P1
Unit cell:
a: 59.67Å b: 73.14Å c: 83.55Å
α: 65.06° β: 71.95° γ: 69.76°
R-values:
R R work R free
0.169 0.169 0.2
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Microbial degradation of complex carbohydrates in the gut.
Flint et al. (2012)
16 more

2 mentions without citation

Complex glycan catabolism by the human gut microbiota: the Bacteroidetes Sus-like paradigm.
Martens et al. (2009)
1 more