› 3cj8

X-ray diffraction
1.95Å resolution

Crystal structure of 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase from Enterococcus faecalis V583

Released:
DOI: 10.2210/pdb3cj8/pdb
PDB entry 3cj8 coloured by chain, front view.
PDB entry 3cj8 coloured by chain, side view.
PDB entry 3cj8 coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry authors: Tan K, Bigelow L, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H(2)O = CoA + L-2-acetamido-6-oxoheptanedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 236 amino acids
Theoretical weight: 25.15 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q836H8 (Residues: 2-233; Coverage: 100%)
Gene names: EF_1133, dapH
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand CL 6 x CL
Ligand ACT 1 x ACT
Ligand FMT 7 x FMT
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 95.209Å b: 55.179Å c: 140.387Å
α: 90° β: 95.63° γ: 90°
R-values:
R R work R free
0.204 0.201 0.248
Expression system: Escherichia coli BL21

Quick links