› 3bzx

X-ray diffraction
1.6Å resolution

Crystal structure of the mutated H265A EscU C-terminal domain

Released:
DOI: 10.2210/pdb3bzx/pdb
PDB entry 3bzx coloured by chain, front view.
PDB entry 3bzx coloured by chain, side view.
PDB entry 3bzx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.
Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC
Nature 453 124-7 (2008)
PMID: 18451864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189559 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
EscU protein Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 5.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 215-262; Coverage: 14%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, EAN77_23705, EAX79_25000, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
EscU protein Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 263-345; Coverage: 24%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, EAN77_23705, EAX79_25000, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
Structure domains: secretion proteins EscU

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 55.739Å b: 59.024Å c: 72.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
28 more