› 3bzt

X-ray diffraction
1.5Å resolution

Crystal structural of the mutated P263A EscU C-terminal domain

Released:
DOI: 10.2210/pdb3bzt/pdb
PDB entry 3bzt coloured by chain, front view.
PDB entry 3bzt coloured by chain, side view.
PDB entry 3bzt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.
Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC
Nature 453 124-7 (2008)
PMID: 18451864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T3SS structure protein EscU Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 215-345; Coverage: 38%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, EAN77_23705, EAX79_25000, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
Sequence domains: FlhB HrpN YscU SpaS Family
Structure domains: secretion proteins EscU

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I222
Unit cell:
a: 56.411Å b: 59.091Å c: 72.105Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
28 more