› 3bxg

X-ray diffraction
1.8Å resolution

Crystal structure of effector binding domain of central glycolytic gene regulator (CggR) from Bacillus subtilis in complex with glucose-6-phosphate

Released:
DOI: 10.2210/pdb3bxg/pdb
PDB entry 3bxg coloured by chain, front view.
PDB entry 3bxg coloured by chain, side view.
PDB entry 3bxg coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová P, Kozísek M, Moy SF, Sieglová I, Joachimiak A, Machius M, Otwinowski Z
Mol Microbiol 69 895-910 (2008)
PMID: 18554327

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Central glycolytic genes regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.56 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32253 (Residues: 89-340; Coverage: 74%)
Gene names: BSU33950, cggR, yvbQ
Sequence domains: Putative sugar-binding domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BG6 2 x BG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 55.494Å b: 83.806Å c: 113.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.233
Expression system: Escherichia coli

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Citations

3 review citations

The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes.
Boeri Erba et al. (2015)
2 more

1 mention without citation

Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová et al. (2008)