3bx3

X-ray diffraction
3Å resolution

Puf4 T650C/C724R Mutant bound to Cox17 RNA 3' UTR recognition sequence

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-150435 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homology domain family member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25339 (Residues: 554-888; Coverage: 38%)
Gene names: PUF4, YGL014W, YGL023
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
COX17 RNA target sequence Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 66.065Å b: 137.116Å c: 160.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.29 0.35
Expression systems:
  • Escherichia coli
  • Not provided