› 3bsz

X-ray diffraction
3.38Å resolution

Crystal structure of the transthyretin-retinol binding protein-Fab complex

Released:
DOI: 10.2210/pdb3bsz/pdb
PDB entry 3bsz coloured by chain, front view.
PDB entry 3bsz coloured by chain, side view.
PDB entry 3bsz coloured by chain, top view.
Source organisms:
Primary publication:
Structural and mutational analyses of protein-protein interactions between transthyretin and retinol-binding protein.
Zanotti G, Folli C, Cendron L, Alfieri B, Nishida SK, Gliubich F, Pasquato N, Negro A, Berni R
FEBS J 275 5841-54 (2008)
PMID: 19021760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-233908 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Transthyretin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain
Retinol-binding protein 4 Chains: E, F
Molecule details ›
Chains: E, F
Length: 176 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P02753 (Residues: 19-194; Coverage: 96%)
Gene names: PRO2222, RBP4
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin
Fab fragment heavy chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 215 amino acids
Theoretical weight: 23.71 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Fab fragment light chain Chains: H, N
Molecule details ›
Chains: H, N
Length: 215 amino acids
Theoretical weight: 22.94 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand RTL 2 x RTL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C222
Unit cell:
a: 159.62Å b: 223.18Å c: 121.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.312
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

A Narrative Review of the Role of Transthyretin in Health and Disease.
Liz et al. (2020)
2 more

8 mentions without citation

The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.
Widjaja-Adhi et al. (2020)
7 more