› 3bor

X-ray diffraction
1.85Å resolution

Crystal structure of the DEADc domain of human translation initiation factor 4A-2

Released:
DOI: 10.2210/pdb3bor/pdb
PDB entry 3bor coloured by chain, front view.
PDB entry 3bor coloured by chain, side view.
PDB entry 3bor coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative structural analysis of human DEAD-box RNA helicases.
Schütz P, Karlberg T, van den Berg S, Collins R, Lehtiö L, Högbom M, Holmberg-Schiavone L, Tempel W, Park HW, Hammarström M, Moche M, Thorsell AG, Schüler H
PLoS One 5 (2010)
PMID: 20941364

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic initiation factor 4A-II Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14240 (Residues: 22-240; Coverage: 54%)
Gene names: DDX2B, EIF4A2, EIF4F
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 58.093Å b: 80.102Å c: 42.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Dbp5, Gle1-IP6 and Nup159: a working model for mRNP export.
Folkmann et al. (2011)
16 more

4 mentions without citation

Comparative structural analysis of human DEAD-box RNA helicases.
Schütz et al. (2010)
3 more