› 3bol

X-ray diffraction
1.85Å resolution

Cobalamin-dependent methionine synthase (1-566) from Thermotoga maritima complexed with Zn2+

Released:
DOI: 10.2210/pdb3bol/pdb
PDB entry 3bol coloured by chain, front view.
PDB entry 3bol coloured by chain, side view.
PDB entry 3bol coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Metal active site elasticity linked to activation of homocysteine in methionine synthases.
Koutmos M, Pejchal R, Bomer TM, Matthews RG, Smith JL, Ludwig ML
Proc Natl Acad Sci U S A 105 3286-91 (2008)
PMID: 18296644

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 566 amino acids
Theoretical weight: 63.63 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYA5 (Residues: 1-566; Coverage: 74%)
Gene name: TM_0268
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 3 x ZN
Ligand K 2 x K
Ligand YT3 1 x YT3
Ligand HCS 1 x HCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 59.626Å b: 85.841Å c: 125.587Å
α: 90° β: 100.67° γ: 90°
R-values:
R R work R free
0.195 0.193 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cobalamin-dependent and cobamide-dependent methyltransferases.
Matthews et al. (2008)
4 more

2 mentions without citation

Metal active site elasticity linked to activation of homocysteine in methionine synthases.
Koutmos et al. (2008)
1 more