› 3b9a

X-ray diffraction
1.8Å resolution

Crystal structure of Vibrio harveyi chitinase A complexed with hexasaccharide

Released:
DOI: 10.2210/pdb3b9a/pdb
PDB entry 3b9a coloured by chain, front view.
PDB entry 3b9a coloured by chain, side view.
PDB entry 3b9a coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism.
Songsiriritthigul C, Pantoom S, Aguda AH, Robinson RC, Suginta W
J Struct Biol 162 491-9 (2008)
PMID: 18467126

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 584 amino acids
Theoretical weight: 63.84 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AMP1 (Residues: 22-597; Coverage: 70%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 63.704Å b: 83.323Å c: 106.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.21
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
5 more

5 mentions without citation

Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding.
Li et al. (2010)
4 more