› 3b8z

X-ray diffraction
1.4Å resolution

High Resolution Crystal Structure of the Catalytic Domain of ADAMTS-5 (Aggrecanase-2)

Released:
DOI: 10.2210/pdb3b8z/pdb
PDB entry 3b8z coloured by chain, front view.
PDB entry 3b8z coloured by chain, side view.
PDB entry 3b8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structure of the catalytic domain of ADAMTS-5 (aggrecanase-2).
Shieh HS, Mathis KJ, Williams JM, Hills RL, Wiese JF, Benson TE, Kiefer JR, Marino MH, Carroll JN, Leone JW, Malfait AM, Arner EC, Tortorella MD, Tomasselli A
J Biol Chem 283 1501-1507 (2008)
PMID: 17991750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
A disintegrin and metalloproteinase with thrombospondin motifs 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA0 (Residues: 264-480; Coverage: 24%)
Gene names: ADAMTS11, ADAMTS5, ADMP2
Sequence domains: Reprolysin (M12B) family zinc metalloprotease
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 6 x CA
Ligand 294 2 x 294
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 53.057Å b: 44.487Å c: 76.38Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.187 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Unraveling the scissile bond: how ADAMTS13 recognizes and cleaves von Willebrand factor.
Crawley et al. (2011)
8 more

5 mentions without citation

ADAM and ADAMTS Family Proteins and Snake Venom Metalloproteinases: A Structural Overview.
Takeda S. (2016)
4 more