› 3b8o

X-ray diffraction
2.4Å resolution

Structure of WzzE- Bacterial Polysaccharide Co-polymerase

Released:
DOI: 10.2210/pdb3b8o/pdb
PDB entry 3b8o coloured by chain, front view.
PDB entry 3b8o coloured by chain, side view.
PDB entry 3b8o coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length.
Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Féthière J, Matte A, Cygler M
Nat Struct Mol Biol 15 130-8 (2008)
PMID: 18204465

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ECA polysaccharide chain length modulation protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 265 amino acids
Theoretical weight: 30.91 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG01 (Residues: 54-318; Coverage: 76%)
Gene names: ECs4718, Z5296, wzz, wzzE
Sequence domains: Chain length determinant protein
Structure domains: FepE-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 88 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 107.438Å b: 108.299Å c: 122.569Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.225 0.223 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Pivotal roles of the outer membrane polysaccharide export and polysaccharide copolymerase protein families in export of extracellular polysaccharides in gram-negative bacteria.
Cuthbertson et al. (2009)
17 more

8 mentions without citation

Biochemical and structural analysis of bacterial O-antigen chain length regulator proteins reveals a conserved quaternary structure.
Larue et al. (2009)
7 more