› 3aou

X-ray diffraction
3.14Å resolution

Structure of the Na+ unbound rotor ring modified with N,N f-Dicyclohexylcarbodiimide of the Na+-transporting V-ATPase

Released:
DOI: 10.2210/pdb3aou/pdb
PDB entry 3aou coloured by chain, front view.
PDB entry 3aou coloured by chain, side view.
PDB entry 3aou coloured by chain, top view.
Source organism: Enterococcus hirae
Primary publication:
Structure of the rotor ring modified with N,N'-dicyclohexylcarbodiimide of the Na+-transporting vacuolar ATPase.
Mizutani K, Yamamoto M, Suzuki K, Yamato I, Kakinuma Y, Shirouzu M, Walker JE, Yokoyama S, Iwata S, Murata T
Proc Natl Acad Sci U S A 108 13474-9 (2011)
PMID: 21813759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V-type sodium ATPase subunit K Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 156 amino acids
Theoretical weight: 16.04 KDa
Source organism: Enterococcus hirae
Expression system: Enterococcus hirae
UniProt:
  • Canonical: P43457 (Residues: 1-156; Coverage: 100%)
Gene names: EHR_08240, ntpK, ntpN
Sequence domains: ATP synthase subunit C
Structure domains: lithium bound rotor ring of v- atpase

Ligands and Environments

2 bound ligands:
Ligand DCW 10 x DCW
Ligand UMQ 2 x UMQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 120.41Å b: 124.937Å c: 208.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.248
Expression system: Enterococcus hirae

Quick links

Citations

4 review citations

Rotary ATPases--dynamic molecular machines.
Stewart et al. (2014)
3 more

3 mentions without citation

Structure of the rotor ring modified with N,N'-dicyclohexylcarbodiimide of the Na+-transporting vacuolar ATPase.
Mizutani et al. (2011)
2 more