3afi

X-ray diffraction
1.75Å resolution

Function and Biology Details

Reaction catalysed:
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-157796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 316 amino acids
Theoretical weight: 34.96 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: P59337 (Residues: 1-310; Coverage: 100%)
Gene names: blr1087, dhaA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 212.855Å b: 117.843Å c: 55.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.191
Expression system: Escherichia coli