› 2zib

X-ray diffraction
1.34Å resolution

Crystal structure analysis of calcium-independent type II antifreeze protein

Released:
DOI: 10.2210/pdb2zib/pdb
PDB entry 2zib coloured by chain, front view.
PDB entry 2zib coloured by chain, side view.
PDB entry 2zib coloured by chain, top view.
Source organism: Brachyopsis segaliensis
Primary publication:
Crystal structure and mutational analysis of Ca2+-independent type II antifreeze protein from longsnout poacher, Brachyopsis rostratus.
Nishimiya Y, Kondo H, Takamichi M, Sugimoto H, Suzuki M, Miura A, Tsuda S
J Mol Biol 382 734-46 (2008)
PMID: 18674542

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 ice-structuring protein Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.52 KDa
Source organism: Brachyopsis segaliensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0ZT93 (Residues: 42-168; Coverage: 84%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 43.275Å b: 48.416Å c: 59.707Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.131 0.164
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Ice-Binding Proteins and Their Function.
Bar Dolev et al. (2016)
5 more

7 mentions without citation

Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.
Białkowska et al. (2020)
6 more