2z3x

X-ray diffraction
2.1Å resolution

Structure of a Protein-DNA Complex Essential for DNA Protection in Spore of Bacillus Species

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-115959 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Small, acid-soluble spore protein C Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 63 amino acids
Theoretical weight: 6.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02958 (Residues: 13-72; Coverage: 83%)
Gene names: BSU19950, sspC
Sequence domains: Small, acid-soluble spore proteins, alpha/beta type
Structure domains: Small, acid-soluble spore protein, alpha/beta type-like
5'-D(*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.56 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6122
Unit cell:
a: 86.962Å b: 86.962Å c: 144.669Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.257 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided