› 2xnr

X-ray diffraction
1.6Å resolution

Structural insights into cis element recognition of non- polyadenylated RNAs by the Nab3-RRM

Released:
DOI: 10.2210/pdb2xnr/pdb
PDB entry 2xnr coloured by chain, front view.
PDB entry 2xnr coloured by chain, side view.
PDB entry 2xnr coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insights into cis element recognition of non-polyadenylated RNAs by the Nab3-RRM.
Lunde BM, Hörner M, Meinhart A
Nucleic Acids Res 39 337-46 (2011)
PMID: 20805243

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153794 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nuclear polyadenylated RNA-binding protein 3 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38996 (Residues: 329-404; Coverage: 10%)
Gene names: HMD1, NAB3, YPL190C
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*UP*UP*CP*UP*UP*AP*UP*UP*CP*UP*UP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 30.64Å b: 30.64Å c: 82.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.184
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Termination of Transcription of Short Noncoding RNAs by RNA Polymerase II.
Arndt et al. (2015)
3 more

5 mentions without citation

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency.
Hempel et al. (2012)
4 more