› 2q2v

X-ray diffraction
1.9Å resolution

Structure of D-3-Hydroxybutyrate Dehydrogenase from Pseudomonas putida

Released:
DOI: 10.2210/pdb2q2v/pdb
PDB entry 2q2v coloured by chain, front view.
PDB entry 2q2v coloured by chain, side view.
PDB entry 2q2v coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Cosubstrate-induced dynamics of D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida.
Paithankar KS, Feller C, Kuettner EB, Keim A, Grunow M, Sträter N
FEBS J 274 5767-79 (2007)
PMID: 17958702

Function and Biology Details

Reaction catalysed: 
(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-D-hydroxybutyrate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 26.51 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AE70 (Residues: 2-256; Coverage: 100%)
Gene name: bdhA
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 115.328Å b: 58.176Å c: 119.382Å
α: 90° β: 92.3° γ: 90°
R-values:
R R work R free
0.171 0.169 0.219
Expression system: Escherichia coli

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Citations

13 citation in other articles

A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts.
Yeom et al. (2018)
12 more