› 2pms

X-ray diffraction
2.91Å resolution

Crystal structure of the complex of human lactoferrin N-lobe and lactoferrin-binding domain of pneumococcal surface protein A

Released:
DOI: 10.2210/pdb2pms/pdb
PDB entry 2pms coloured by chain, front view.
PDB entry 2pms coloured by chain, side view.
PDB entry 2pms coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a complex of human lactoferrin N-lobe with pneumococcal surface protein a provides insight into microbial defense mechanism.
Senkovich O, Cook WJ, Mirza S, Hollingshead SK, Protasevich II, Briles DE, Chattopadhyay D
J Mol Biol 370 701-13 (2007)
PMID: 17543335

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136231 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lactotransferrin Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 38.28 KDa
Source organism: Homo sapiens
Expression system: Insect cells
UniProt:
  • Canonical: P02788 (Residues: 21-362; Coverage: 50%)
Gene names: GIG12, LF, LTF
Sequence domains: Transferrin
Structure domains: Periplasmic binding protein-like II
Pneumococcal surface protein A Chains: C, D
Molecule details ›
Chains: C, D
Length: 125 amino acids
Theoretical weight: 14.12 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q54972 (Residues: 199-319; Coverage: 20%)
Gene name: pspA
Structure domains: Helix Hairpins

Ligands and Environments

5 bound ligands:
Ligand NAG 2 x NAG
Ligand CO3 2 x CO3
Ligand FE 2 x FE
Ligand SO4 6 x SO4
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P32
Unit cell:
a: 130.18Å b: 130.18Å c: 80.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.249
Expression systems:
  • Insect cells
  • Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Pathogenesis and pathophysiology of pneumococcal meningitis.
Mook-Kanamori et al. (2011)
14 more

6 mentions without citation

Choline Binding Proteins from Streptococcus pneumoniae: A Dual Role as Enzybiotics and Targets for the Design of New Antimicrobials.
Maestro et al. (2016)
5 more