› 2zxl

X-ray diffraction
2.4Å resolution

Crystal structure of red chlorophyll catabolite reductase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2zxl/pdb
PDB entry 2zxl coloured by chain, front view.
PDB entry 2zxl coloured by chain, side view.
PDB entry 2zxl coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of red chlorophyll catabolite reductase: enlargement of the ferredoxin-dependent bilin reductase family.
Sugishima M, Kitamori Y, Noguchi M, Kohchi T, Fukuyama K
J Mol Biol 389 376-87 (2009)
PMID: 19374909

Function and Biology Details

Reaction catalysed: 
Primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster = red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red chlorophyll catabolite reductase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.48 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8LDU4 (Residues: 40-319; Coverage: 88%)
Gene names: ACD2, At4g37000, C7A10_360, RCCR
Sequence domains: Red chlorophyll catabolite reductase (RCC reductase)
Structure domains: oxygen-dependent coproporphyrinogen oxidase

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 52.286Å b: 68.14Å c: 83.273Å
α: 90° β: 95.32° γ: 90°
R-values:
R R work R free
0.221 0.216 0.298
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Plant type ferredoxins and ferredoxin-dependent metabolism.
Hanke et al. (2013)
4 more