› 2zwp

X-ray diffraction
2.4Å resolution

Crystal structure of Ca3 site mutant of Pro-S324A

Released:
DOI: 10.2210/pdb2zwp/pdb
PDB entry 2zwp coloured by chain, front view.
PDB entry 2zwp coloured by chain, side view.
PDB entry 2zwp coloured by chain, top view.
Source organism: Thermococcus kodakarensis
Primary publication:
Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon.
Takeuchi Y, Tanaka S, Matsumura H, Koga Y, Takano K, Kanaya S
Biochemistry 48 10637-43 (2009)
PMID: 19813760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tk-subtilisin Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 41.35 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58502 (Residues: 25-422; Coverage: 100%)
Gene name: TK1675
Sequence domains: Subtilase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 11 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 50.575Å b: 87.736Å c: 155.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Calcium binding proteins and calcium signaling in prokaryotes.
Domínguez et al. (2015)
1 more