› 2zwk

X-ray diffraction
3.1Å resolution

Crystal structure of intimin-Tir90 complex

Released:
DOI: 10.2210/pdb2zwk/pdb
PDB entry 2zwk coloured by chain, front view.
PDB entry 2zwk coloured by chain, side view.
PDB entry 2zwk coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. EDL933
Entry authors: Ma Y, Gao F, Li D-F, Gao GF

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155017 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Intimin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 184 amino acids
Theoretical weight: 20.17 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43261 (Residues: 752-934; Coverage: 20%)
Gene names: ECs4559, L0025, Z5110, eae, eaeA
Sequence domains:
Structure domains:
Translocated intimin receptor Tir Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 72 amino acids
Theoretical weight: 7.99 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DB77 (Residues: 274-336; Coverage: 11%)
Gene names: ECs4561, Z5112, espE, tir
Sequence domains: Translocated intimin receptor (Tir) intimin-binding domain
Structure domains: Translocated intimin receptor, central domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I4122
Unit cell:
a: 96.78Å b: 96.78Å c: 644.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.301 0.36
Expression system: Escherichia coli BL21(DE3)

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