› 2zvl

X-ray diffraction
2.5Å resolution

Crystal structure of PCNA in complex with DNA polymerase kappa fragment

Released:
DOI: 10.2210/pdb2zvl/pdb
PDB entry 2zvl coloured by chain, front view.
PDB entry 2zvl coloured by chain, side view.
PDB entry 2zvl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for novel interactions between human translesion synthesis polymerases and proliferating cell nuclear antigen.
Hishiki A, Hashimoto H, Hanafusa T, Kamei K, Ohashi E, Shimizu T, Ohmori H, Sato M
J Biol Chem 284 10552-60 (2009)
PMID: 19208623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146051 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase kappa Chains: U, V, W, X, Y, Z
Molecule details ›
Chains: U, V, W, X, Y, Z
Length: 14 amino acids
Theoretical weight: 1.7 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P1
Unit cell:
a: 74.769Å b: 74.707Å c: 109.049Å
α: 87.25° β: 77.46° γ: 80.26°
R-values:
R R work R free
0.229 0.225 0.289
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Regulation of PCNA-protein interactions for genome stability.
Mailand et al. (2013)
19 more

12 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
11 more