› 2zqk

X-ray diffraction
2.8Å resolution

Crystal structure of intimin-Tir68 complex

Released:
DOI: 10.2210/pdb2zqk/pdb
PDB entry 2zqk coloured by chain, front view.
PDB entry 2zqk coloured by chain, side view.
PDB entry 2zqk coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Entry authors: Ma Y, Gao F, Li D-F, Gao GF

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155015 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Intimin Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 20.67 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43261 (Residues: 747-934; Coverage: 20%)
Gene names: ECs4559, L0025, Z5110, eae, eaeA
Sequence domains:
Structure domains:
Translocated intimin receptor Tir Chains: C, D, M, N
Molecule details ›
Chains: C, D, M, N
Length: 77 amino acids
Theoretical weight: 8.4 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DB77 (Residues: 269-336; Coverage: 12%)
Gene names: ECs4561, Z5112, espE, tir
Sequence domains: Translocated intimin receptor (Tir) intimin-binding domain
Structure domains: Translocated intimin receptor, central domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 116.68Å b: 42.31Å c: 182.08Å
α: 90° β: 101.13° γ: 90°
R-values:
R R work R free
0.241 0.241 0.3
Expression system: Escherichia coli BL21(DE3)

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