› 2zmz

X-ray diffraction
1.37Å resolution

The 1.37-A crystal structure of the hydroxylamine-induced deoxy-form of the copper-bound tyrosinase in complex with a caddie protein from Streptomyces castaneoglobisporus

Released:
DOI: 10.2210/pdb2zmz/pdb
PDB entry 2zmz coloured by chain, front view.
PDB entry 2zmz coloured by chain, side view.
PDB entry 2zmz coloured by chain, top view.
Source organism: Streptomyces castaneoglobisporus
Entry authors: Matoba Y, Sugiyama M

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosinase copper-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.09 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS2 (Residues: 1-273; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase
MelC Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.2 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS1 (Residues: 1-126; Coverage: 100%)
Sequence domains: Tyrosinase co-factor MelC1
Structure domains: protein ne1242 domain like

Ligands and Environments

2 bound ligands:
Ligand CU1 3 x CU1
Ligand NO3 6 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 65.18Å b: 98.03Å c: 55.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.232
Expression system: Escherichia coli

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