› 2zjv

X-ray diffraction
2.7Å resolution

Crystal Structure of Lymnaea stagnalis Acetylcholine Binding Protein (Ls-AChBP) Complexed with Clothianidin

Released:
DOI: 10.2210/pdb2zjv/pdb
PDB entry 2zjv coloured by chain, front view.
PDB entry 2zjv coloured by chain, side view.
PDB entry 2zjv coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin.
Ihara M, Okajima T, Yamashita A, Oda T, Hirata K, Nishiwaki H, Morimoto T, Akamatsu M, Ashikawa Y, Kuroda S, Mega R, Kuramitsu S, Sattelle DB, Matsuda K
Invert Neurosci 8 71-81 (2008)
PMID: 18338186

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 214 amino acids
Theoretical weight: 24.22 KDa
Source organism: Lymnaea stagnalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P58154 (Residues: 16-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand CT4 5 x CT4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P65
Unit cell:
a: 74.564Å b: 74.564Å c: 351.049Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 0.27
Expression system: Komagataella pastoris

Quick links

Citations

10 review citations

Nicotinic receptors: allosteric transitions and therapeutic targets in the nervous system.
Taly et al. (2009)
9 more

6 mentions without citation

Size matters in activation/inhibition of ligand-gated ion channels.
Du et al. (2012)
5 more