› 2zj7

X-ray diffraction
2.21Å resolution

Crystal structure of D157A mutant of Pseudomonas sp. MIS38 lipase

Released:
DOI: 10.2210/pdb2zj7/pdb
PDB entry 2zj7 coloured by chain, front view.
PDB entry 2zj7 coloured by chain, side view.
PDB entry 2zj7 coloured by chain, top view.
Source organism: Pseudomonas sp. MIS38
Primary publication:
Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability.
Kuwahara K, Angkawidjaja C, Matsumura H, Koga Y, Takano K, Kanaya S
Protein Eng Des Sel 21 737-44 (2008)
PMID: 18987131

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 617 amino acids
Theoretical weight: 64.52 KDa
Source organism: Pseudomonas sp. MIS38
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RBY1 (Residues: 1-617; Coverage: 100%)
Sequence domains: RTX calcium-binding nonapeptide repeat (4 copies)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CA 10 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 48.634Å b: 84.627Å c: 87.521Å
α: 90° β: 96.85° γ: 90°
R-values:
R R work R free
0.168 0.164 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metals and breast cancer.
Byrne et al. (2013)
1 more

1 mention without citation

Generalized Approach towards Secretion-Based Protein Production via Neutralization of Secretion-Preventing Cationic Substrate Residues.
Byun et al. (2022)