› 2zag

X-ray diffraction
3Å resolution

Crystal structure of the SeMet-substituted soluble domain of STT3 from P. furiosus

Released:
DOI: 10.2210/pdb2zag/pdb
PDB entry 2zag coloured by chain, front view.
PDB entry 2zag coloured by chain, side view.
PDB entry 2zag coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structure-guided identification of a new catalytic motif of oligosaccharyltransferase.
Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D
EMBO J 27 234-43 (2008)
PMID: 18046457

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-186491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichyl-phosphooligosaccharide-protein glycotransferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 497 amino acids
Theoretical weight: 56.21 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8U4D2 (Residues: 471-967; Coverage: 51%)
Gene names: PF0156, aglB1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 137.402Å b: 266.397Å c: 73.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.272
Expression system: Escherichia coli BL21

Quick links

Citations

33 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
32 more

4 mentions without citation

Glycosyltransferase-mediated Sweet Modification in Oral Streptococci.
Zhu et al. (2015)
3 more