› 2z5h

X-ray diffraction
2.89Å resolution

Crystal structure of the head-to-tail junction of tropomyosin complexed with a fragment of TnT

Released:
DOI: 10.2210/pdb2z5h/pdb
PDB entry 2z5h coloured by chain, front view.
PDB entry 2z5h coloured by chain, side view.
PDB entry 2z5h coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of a molecular swivel by troponin-T.
Murakami K, Stewart M, Nozawa K, Tomii K, Kudou N, Igarashi N, Shirakihara Y, Wakatsuki S, Yasunaga T, Wakabayashi T
Proc Natl Acad Sci U S A 105 7200-5 (2008)
PMID: 18483193

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-238682 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
General control transcription factor GCN4; Tropomyosin alpha-1 chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 259-278; Coverage: 7%)
  • Canonical: P58772 (Residues: 254-284; Coverage: 11%)
Gene names: AAS101, AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
General control transcription factor GCN4; Tropomyosin alpha-1 chain Chain: I
Molecule details ›
Chain: I
Length: 40 amino acids
Theoretical weight: 4.54 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 267-278; Coverage: 4%)
  • Canonical: P58772 (Residues: 1-24; Coverage: 9%)
Gene names: AAS101, AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Troponin T, fast skeletal muscle isoforms Chain: T
Molecule details ›
Chain: T
Length: 55 amino acids
Theoretical weight: 6.67 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12620 (Residues: 58-112; Coverage: 21%)
Gene name: TNNT3
Sequence domains: Troponin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I212121
Unit cell:
a: 86.809Å b: 158.308Å c: 163.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.247
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The streptococcal M protein: a highly versatile molecule.
Smeesters et al. (2010)
13 more

13 mentions without citation

Thin filament mutations: developing an integrative approach to a complex disorder.
Tardiff JC. (2011)
12 more