› 2ykp

X-ray diffraction
3.1Å resolution

Structure of the human LINE-1 ORF1p trimer

Released:
DOI: 10.2210/pdb2ykp/pdb
PDB entry 2ykp coloured by chain, front view.
PDB entry 2ykp coloured by chain, side view.
PDB entry 2ykp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition.
Khazina E, Truffault V, Büttner R, Schmidt S, Coles M, Weichenrieder O
Nat Struct Mol Biol 18 1006-14 (2011)
PMID: 21822284

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LINE-1 retrotransposable element ORF1 protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 229 amino acids
Theoretical weight: 27.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UN81 (Residues: 104-326; Coverage: 66%)
Gene names: L1RE1, LRE1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 73.73Å b: 89.92Å c: 111.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Roles for retrotransposon insertions in human disease.
Hancks et al. (2016)
14 more

2 mentions without citation

Human LINE-1 retrotransposition requires a metastable coiled coil and a positively charged N-terminus in L1ORF1p.
Khazina et al. (2018)
1 more