› 2y8k

X-ray diffraction
1.47Å resolution

Structure of CtGH5-CBM6, an arabinoxylan-specific xylanase.

Released:
DOI: 10.2210/pdb2y8k/pdb
PDB entry 2y8k coloured by chain, front view.
PDB entry 2y8k coloured by chain, side view.
PDB entry 2y8k coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Structure and function of an arabinoxylan-specific xylanase.
Correia MA, Mazumder K, Brás JL, Firbank SJ, Zhu Y, Lewis RJ, York WS, Fontes CM, Gilbert HJ
J Biol Chem 286 22510-20 (2011)
PMID: 21378160

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate binding family 6 Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 54.16 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DHG6 (Residues: 37-516; Coverage: 51%)
Gene name: Cthe_2193
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 8 x GOL
Ligand CA 1 x CA
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04, ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 69.12Å b: 75.55Å c: 105.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.166
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Thermophilic lignocellulose deconstruction.
Blumer-Schuette et al. (2014)
8 more

8 mentions without citation

Structure and function of an arabinoxylan-specific xylanase.
Correia et al. (2011)
7 more