› 2y3f

X-ray diffraction
1.49Å resolution

Traptavidin, biotin bound form

Released:
DOI: 10.2210/pdb2y3f/pdb
PDB entry 2y3f coloured by chain, front view.
PDB entry 2y3f coloured by chain, side view.
PDB entry 2y3f coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer.
Chivers CE, Koner AL, Lowe ED, Howarth M
Biochem J 435 55-63 (2011)
PMID: 21241253

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.17 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BTN 1 x BTN
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P42212
Unit cell:
a: 57.34Å b: 57.34Å c: 77.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.135 0.152
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis.
Jain et al. (2017)
5 more

3 mentions without citation

How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer.
Chivers et al. (2011)
2 more