› 2xu9

X-ray diffraction
1.5Å resolution

Crystal structure of Laccase from Thermus thermophilus HB27

Released:
DOI: 10.2210/pdb2xu9/pdb
PDB entry 2xu9 coloured by chain, front view.
PDB entry 2xu9 coloured by chain, side view.
PDB entry 2xu9 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
X-ray-induced catalytic active-site reduction of a multicopper oxidase: structural insights into the proton-relay mechanism and O2-reduction states.
Serrano-Posada H, Centeno-Leija S, Rojas-Trejo SP, Rodríguez-Almazán C, Stojanoff V, Rudiño-Piñera E
Acta Crystallogr D Biol Crystallogr 71 2396-411 (2015)
PMID: 26627648

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 48.79 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q72HW2 (Residues: 24-462; Coverage: 95%)
Gene name: TT_C1370
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand CU 4 x CU
Ligand MPD 6 x MPD
Ligand MRD 10 x MRD
Ligand OH 1 x OH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 93.578Å b: 110.339Å c: 96.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.178
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Radiation chemists look at damage in redox proteins induced by X-rays.
Wherland et al. (2018)
1 more

7 mentions without citation

Reciprocal carbonyl-carbonyl interactions in small molecules and proteins.
Rahim et al. (2017)
6 more