› 2xro

X-ray diffraction
3.4Å resolution

Crystal structure of TtgV in complex with its DNA operator

Released:
DOI: 10.2210/pdb2xro/pdb
PDB entry 2xro coloured by chain, front view.
PDB entry 2xro coloured by chain, side view.
PDB entry 2xro coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of TtgV in complex with its DNA operator reveals a general model for cooperative DNA binding of tetrameric gene regulators.
Lu D, Fillet S, Meng C, Alguel Y, Kloppsteck P, Bergeron J, Krell T, Gallegos MT, Ramos J, Zhang X
Genes Dev 24 2556-65 (2010)
PMID: 21078819

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115938 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional regulator TtgV Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 241 amino acids
Theoretical weight: 25.61 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93PU6 (Residues: 14-253; Coverage: 93%)
Gene name: ttgV
Sequence domains:
Structure domains:
TTGV OPERATOR DNA Chain: X
Molecule details ›
Chain: X
Length: 42 nucleotides
Theoretical weight: 12.82 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
TTGV OPERATOR DNA Chain: Y
Molecule details ›
Chain: Y
Length: 43 nucleotides
Theoretical weight: 13.34 KDa
Source organism: Pseudomonas putida
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand OS 2 x OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P65
Unit cell:
a: 89.584Å b: 89.584Å c: 416.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Solvent tolerance in Gram-negative bacteria.
Segura et al. (2012)
3 more

7 mentions without citation

Studying protein-DNA interactions using atomic force microscopy.
Beckwitt et al. (2018)
6 more