› 2xm4

X-ray diffraction
1.1Å resolution

Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124E variant

Released:
DOI: 10.2210/pdb2xm4/pdb
PDB entry 2xm4 coloured by chain, front view.
PDB entry 2xm4 coloured by chain, side view.
PDB entry 2xm4 coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Distal-to-proximal NO conversion in hemoproteins: the role of the proximal pocket.
Hough MA, Antonyuk SV, Barbieri S, Rustage N, McKay AL, Servid AE, Eady RR, Andrew CR, Hasnain SS
J Mol Biol 405 395-409 (2011)
PMID: 21073879

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c' Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.6 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: P00138 (Residues: 1-127; Coverage: 100%)
Sequence domains: Cytochrome C'
Structure domains: Cytochrome c/b562

Ligands and Environments

1 bound ligand:
Ligand HEC 1 x HEC
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P6522
Unit cell:
a: 53.463Å b: 53.463Å c: 180.813Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.17
Expression system: Escherichia coli

Quick links

Citations

4 review citations

How do heme-protein sensors exclude oxygen? Lessons learned from cytochrome c', Nostoc puntiforme heme nitric oxide/oxygen-binding domain, and soluble guanylyl cyclase.
Tsai et al. (2012)
3 more