› 2xig

X-ray diffraction
1.85Å resolution

The structure of the Helicobacter pylori ferric uptake regulator Fur reveals three functional metal binding sites

Released:
DOI: 10.2210/pdb2xig/pdb
PDB entry 2xig coloured by chain, front view.
PDB entry 2xig coloured by chain, side view.
PDB entry 2xig coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
The structure of the Helicobacter pylori ferric uptake regulator Fur reveals three functional metal binding sites.
Dian C, Vitale S, Leonard GA, Bahlawane C, Fauquant C, Leduc D, Muller C, de Reuse H, Michaud-Soret I, Terradot L
Mol Microbiol 79 1260-75 (2011)
PMID: 21208302

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric uptake regulation protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 150 amino acids
Theoretical weight: 17.68 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25671 (Residues: 1-150; Coverage: 100%)
Gene names: HP_1027, fur
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 12 x ZN
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 48.15Å b: 48.29Å c: 72.25Å
α: 83.32° β: 77.82° γ: 87.65°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

New roles for bacterial siderophores in metal transport and tolerance.
Schalk et al. (2011)
12 more

13 mentions without citation

Evolution of Macromolecular Docking Techniques: The Case Study of Nickel and Iron Metabolism in Pathogenic Bacteria.
Musiani et al. (2015)
12 more