› 2xbl

X-ray diffraction
1.62Å resolution

Crystal structure of GmhA from Burkholderia pseudomallei in complex with product

Released:
DOI: 10.2210/pdb2xbl/pdb
PDB entry 2xbl coloured by chain, front view.
PDB entry 2xbl coloured by chain, side view.
PDB entry 2xbl coloured by chain, top view.
Source organism: Burkholderia pseudomallei K96243
Primary publication:
The structure of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei reveals a zinc binding site at the heart of the active site.
Harmer NJ
J Mol Biol 400 379-92 (2010)
PMID: 20447408

Function and Biology Details

Reaction catalysed: 
D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188136 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoheptose isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 198 amino acids
Theoretical weight: 20.92 KDa
Source organism: Burkholderia pseudomallei K96243
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93UJ2 (Residues: 1-197; Coverage: 100%)
Gene names: BPSL2795, gmhA
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand M7P 4 x M7P
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 73.2Å b: 83.68Å c: 126.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 citation in other articles

Characterization of the Burkholderia pseudomallei K96243 capsular polysaccharide I coding region.
Cuccui et al. (2012)
10 more

2 mentions without citation

Crystal Structure and Comparative Sequence Analysis of GmhA from Colwellia psychrerythraea Strain 34H Provides Insight into Functional Similarity with DiaA.
Do et al. (2015)
1 more