› 2wy6

X-ray diffraction
3.2Å resolution

Clostridium perfringens alpha-toxin strain NCTC8237 mutant T74I

Released:
DOI: 10.2210/pdb2wy6/pdb
PDB entry 2wy6 coloured by chain, front view.
PDB entry 2wy6 coloured by chain, side view.
PDB entry 2wy6 coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Comparison of a nontoxic variant of Clostridium perfringens α-toxin with the toxic wild-type strain.
Vachieri SG, Clark GC, Alape-Girón A, Flores-Díaz M, Justin N, Naylor CE, Titball RW, Basak AK
Acta Crystallogr D Biol Crystallogr 66 1067-74 (2010)
PMID: 20944240

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 370 amino acids
Theoretical weight: 42.6 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0TV31 (Residues: 29-398; Coverage: 100%)
Gene names: CPF_0042, cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 3 x ZN
Ligand CD 15 x CD
Ligand CA 4 x CA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 107.238Å b: 107.238Å c: 225.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.281
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cadmium-zinc exchange and their binary relationship in the structure of Zn-related proteins: a mini review.
Tang et al. (2014)