› 2ws6

X-ray diffraction
1.5Å resolution

Semi-synthetic analogue of human insulin NMeTyrB26-insulin in hexamer form

Released:
DOI: 10.2210/pdb2ws6/pdb
PDB entry 2ws6 coloured by chain, front view.
PDB entry 2ws6 coloured by chain, side view.
PDB entry 2ws6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues.
Jirácek J, Záková L, Antolíková E, Watson CJ, Turkenburg JP, Dodson GG, Brzozowski AM
Proc Natl Acad Sci U S A 107 1966-70 (2010)
PMID: 20133841

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero hexamer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134195 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
Insulin B chain Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

4 bound ligands:
Ligand IPH 6 x IPH
Ligand CL 2 x CL
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 58.962Å b: 61.927Å c: 47.297Å
α: 90° β: 111.9° γ: 90°
R-values:
R R work R free
0.152 0.15 0.2
Expression system: Not provided

Quick links

Citations

4 review citations

Insulin receptor and cancer.
Belfiore et al. (2011)
3 more

1 mention without citation

Non-equivalent role of inter- and intramolecular hydrogen bonds in the insulin dimer interface.
Antolíková et al. (2011)