› 2wlx

X-ray diffraction
1.9Å resolution

Putative thiosulfate sulfurtransferase YnjE

Released:
DOI: 10.2210/pdb2wlx/pdb
PDB entry 2wlx coloured by chain, front view.
PDB entry 2wlx coloured by chain, side view.
PDB entry 2wlx coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Declercq JP, Smeets A, Depuydt M, Collet JF

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate sulfurtransferase YnjE Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 47.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P78067 (Residues: 24-435; Coverage: 100%)
Gene names: JW5287, b1757, ynjE
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 37.4Å b: 83.43Å c: 131.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.251
Expression system: Escherichia coli BL21

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