› 2wjl

X-ray diffraction
2.15Å resolution

Bacteriorhodopsin mutant E194D

Released:
DOI: 10.2210/pdb2wjl/pdb
PDB entry 2wjl coloured by chain, front view.
PDB entry 2wjl coloured by chain, side view.
PDB entry 2wjl coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Directional proton transfer in membrane proteins achieved through protonated protein-bound water molecules: a proton diode.
Wolf S, Freier E, Potschies M, Hofmann E, Gerwert K
Angew Chem Int Ed Engl 49 6889-93 (2010)
PMID: 20680951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.92 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P63
Unit cell:
a: 61.22Å b: 61.22Å c: 111.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 not available not available
Expression system: Halobacterium salinarum

Quick links

Citations

5 review citations

Channelrhodopsin unchained: structure and mechanism of a light-gated cation channel.
Lórenz-Fonfría et al. (2014)
4 more

6 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
5 more