› 2wd4

X-ray diffraction
1.4Å resolution

Ascorbate Peroxidase as a heme oxygenase: w41A variant product with t-butyl peroxide

Released:
DOI: 10.2210/pdb2wd4/pdb
PDB entry 2wd4 coloured by chain, front view.
PDB entry 2wd4 coloured by chain, side view.
PDB entry 2wd4 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Evidence for heme oxygenase activity in a heme peroxidase.
Badyal SK, Eaton G, Mistry S, Pipirou Z, Basran J, Metcalfe CL, Gumiero A, Handa S, Moody PC, Raven EL
Biochemistry 48 4738-46 (2009)
PMID: 19309109

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ascorbate peroxidase Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.25 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43758 (Residues: 2-250; Coverage: 100%)
Gene name: apx1
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FE 1 x FE
Ligand NA 1 x NA
Ligand SO4 2 x SO4
Ligand TBV 1 x TBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P42212
Unit cell:
a: 82.067Å b: 82.067Å c: 75.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.216
Expression system: Escherichia coli

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Citations

2 citation in other articles

Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure: its unique molecular characterization.
Ishikawa et al. (2010)
1 more