› 2wb4

X-ray diffraction
2.8Å resolution

activated diguanylate cyclase PleD in complex with c-di-GMP

Released:
DOI: 10.2210/pdb2wb4/pdb
PDB entry 2wb4 coloured by chain, front view.
PDB entry 2wb4 coloured by chain, side view.
PDB entry 2wb4 coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Entry authors: Wassmann P, Schirmer T

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator PleD Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 50.38 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GZM2 (Residues: 1-454; Coverage: 100%)
Gene names: CCNA_02546, pleD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BEF 2 x BEF
Ligand MG 3 x MG
Ligand C2E 5 x C2E
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 123.56Å b: 127.43Å c: 88.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.268
Expression system: Escherichia coli BL21(DE3)

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