› 2w8b

X-ray diffraction
1.86Å resolution

Crystal structure of processed TolB in complex with Pal

Released:
DOI: 10.2210/pdb2w8b/pdb
PDB entry 2w8b coloured by chain, front view.
PDB entry 2w8b coloured by chain, side view.
PDB entry 2w8b coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins.
Bonsor DA, Hecht O, Vankemmelbeke M, Sharma A, Krachler AM, Housden NG, Lilly KJ, James R, Moore GR, Kleanthous C
EMBO J 28 2846-57 (2009)
PMID: 19696740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141666 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tol-Pal system protein TolB Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 43.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A855 (Residues: 22-430; Coverage: 100%)
Gene names: JW5100, b0740, tolB
Sequence domains:
Structure domains:
Tol-Pal system protein TolB Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 409 amino acids
Theoretical weight: 43.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A855 (Residues: 22-430; Coverage: 100%)
Gene names: JW5100, b0740, tolB
Sequence domains:
Structure domains:
Peptidoglycan-associated lipoprotein Chains: C, E, G, H
Molecule details ›
Chains: C, E, G, H
Length: 118 amino acids
Theoretical weight: 13.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A912 (Residues: 64-173; Coverage: 72%)
Gene names: JW0731, b0741, excC, pal
Sequence domains: OmpA family
Structure domains: OmpA-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 7 x GOL
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 74.74Å b: 89.24Å c: 90.9Å
α: 86.81° β: 89.81° γ: 68.62°
R-values:
R R work R free
0.194 0.191 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The physiology of bacterial cell division.
Egan et al. (2013)
16 more

4 mentions without citation

The multifarious roles of Tol-Pal in Gram-negative bacteria.
Szczepaniak et al. (2020)
3 more