› 2w7t

X-ray diffraction
2.1Å resolution

Trypanosoma brucei CTPS - glutaminase domain with bound acivicin

Released:
DOI: 10.2210/pdb2w7t/pdb
PDB entry 2w7t coloured by chain, front view.
PDB entry 2w7t coloured by chain, side view.
PDB entry 2w7t coloured by chain, top view.
Source organism: Trypanosoma brucei
Entry authors: Welin M, Moche M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Karlberg T, Kotenyova T, Lehtio L, Nilsson ME, Nyman T, Persson C, Sagemark J, Schueler H, Siponen MI, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Wikstrom M, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.76 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4GZ95 (Residues: 319-589; Coverage: 46%)
Gene name: TB927.1.1240
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand 5CS 1 x 5CS

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 50.48Å b: 63.68Å c: 76.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.262
Expression system: Escherichia coli BL21(DE3)

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