› 2w68

X-ray diffraction
2.5Å resolution

ENHANCING THE RECEPTOR AFFINITY OF THE SIALIC ACID-BINDING DOMAIN OF VIBRIO CHOLERAE SIALIDASE THROUGH MULTIVALENCY

Released:
DOI: 10.2210/pdb2w68/pdb
PDB entry 2w68 coloured by chain, front view.
PDB entry 2w68 coloured by chain, side view.
PDB entry 2w68 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Enhancing the receptor affinity of the sialic acid-binding domain of Vibrio cholerae sialidase through multivalency.
Connaris H, Crocker PR, Taylor GL
J Biol Chem 284 7339-51 (2009)
PMID: 19124471

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sialidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 195 amino acids
Theoretical weight: 21.1 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C6E9 (Residues: 25-216; Coverage: 25%)
Gene names: VC_1784, nanH
Sequence domains: Vibrio cholerae sialidase, lectin insertion
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 138.6Å b: 197.62Å c: 82.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.256
Expression system: Escherichia coli

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Citations

6 review citations

Gateways for the intracellular access of nanocarriers: a review of receptor-mediated endocytosis mechanisms and of strategies in receptor targeting.
Zaki et al. (2010)
5 more

2 mentions without citation

Enhancing the receptor affinity of the sialic acid-binding domain of Vibrio cholerae sialidase through multivalency.
Connaris et al. (2009)
1 more