› 2w5y

X-ray diffraction
2Å resolution

Binary Complex of the Mixed Lineage Leukaemia (MLL1) SET Domain with the cofactor product S-Adenosylhomocysteine.

Released:
DOI: 10.2210/pdb2w5y/pdb
PDB entry 2w5y coloured by chain, front view.
PDB entry 2w5y coloured by chain, side view.
PDB entry 2w5y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the requirement of additional factors for MLL1 SET domain activity and recognition of epigenetic marks.
Southall SM, Wong PS, Odho Z, Roe SM, Wilson JR
Mol Cell 33 181-91 (2009)
PMID: 19187761

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MLL cleavage product C180 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03164 (Residues: 3785-3969; Coverage: 5%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Sequence domains: SET domain
Structure domains: SET domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 48.85Å b: 56.21Å c: 78.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Hijacked in cancer: the KMT2 (MLL) family of methyltransferases.
Rao et al. (2015)
40 more

15 mentions without citation

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Black et al. (2012)
14 more