› 2vzr

X-ray diffraction
1.95Å resolution

C-terminal CBM35 from Amycolatopsis orientalis exo-chitosanase CsxA in complex with glucuronic acid

Released:
DOI: 10.2210/pdb2vzr/pdb
PDB entry 2vzr coloured by chain, front view.
PDB entry 2vzr coloured by chain, side view.
PDB entry 2vzr coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Primary publication:
Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function.
Montanier C, van Bueren AL, Dumon C, Flint JE, Correia MA, Prates JA, Firbank SJ, Lewis RJ, Grondin GG, Ghinet MG, Gloster TM, Herve C, Knox JP, Talbot BG, Turkenburg JP, Kerovuo J, Brzezinski R, Fontes CM, Davies GJ, Boraston AB, Gilbert HJ
Proc Natl Acad Sci U S A 106 3065-70 (2009)
PMID: 19218457

Function and Biology Details

Reaction catalysed: 
Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-beta-D-glucosaminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.03 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56F26 (Residues: 906-1032; Coverage: 13%)
Gene name: csxA
Sequence domains: Carbohydrate binding module (family 35)
Structure domains: Galactose-binding domain-like

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand GCU 2 x GCU
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P212121
Unit cell:
a: 54.569Å b: 56.981Å c: 81.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
14 more

1 mention without citation

Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27.
Okazawa et al. (2015)