2vwt

X-ray diffraction
1.93Å resolution

Crystal structure of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12 - Mg-pyruvate product complex

Released:

Function and Biology Details

Reaction catalysed:
2-dehydro-3-deoxy-L-rhamnonate = pyruvate + (S)-lactaldehyde
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-160093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-keto-3-deoxy-L-rhamnonate aldolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 28.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76469 (Residues: 1-267; Coverage: 100%)
Gene names: JW2239, b2245, rhmA, yfaU
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2221
Unit cell:
a: 105.6Å b: 137.7Å c: 124.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.206
Expression system: Escherichia coli